Rat histidine decarboxylase is a substrate for m-calpain in vitro.
نویسندگان
چکیده
We have followed the in vitro degradation of rat histidine decarboxylase in a reconstituted system, containing only rat histidine decarboxylase (obtained by in vitro transcription and translation), calcium ions in the millimolar range of concentrations, and m-calpain. Under the experimental conditions used, m-calpain quickly and efficiently degraded rat histidine decarboxylase, giving rise to a major proteolytic band of 29 kDa. In a conventional in vitro degradation system containing rabbit reticulocytes supplemented with calcium ions, there was also an intense proteolysis of rat histidine decarboxylase, strongly inhibited in the presence of calpeptin, a highly specific calpain inhibitor.
منابع مشابه
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ورودعنوان ژورنال:
- Biochemical and biophysical research communications
دوره 271 3 شماره
صفحات -
تاریخ انتشار 2000